Table 3 |
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Metaphase FISH screening in human CML cell lines |
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Cell line |
Source |
Cell type |
Signal pattern with RP11-83J21(G) and RP11-323H21(R) probes |
Location of 9q subtelomeric probe |
Location of 22q subtelomeric probe |
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|
|
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|
BV173 |
PB |
B cell precursor |
i. |
2F: der(22)×2[20] |
i. |
der(22)×2 |
i. |
22,der(9) |
|
|
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|
CML-T11 |
PB |
T cell |
i. |
3F: 9,der(22)×2 |
i. |
9,der(9) |
NT |
|
|
ii. |
6F: 9×2,der(22)×4 |
ii. |
9×2,der(9)×2 |
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|
iii. |
4F: der(22)×4 |
iii. |
der(9)×2 |
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|
|
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|
EM-2 |
BM |
Myeloid |
i. |
3F: der(22)×3[1] |
i. |
der(22)×3 |
i. |
22,der(9)×2 |
|
ii. |
4F: der(22)×4[20] |
ii. |
der(22)×4 |
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|
iii. |
5F: der(22)×5[21] |
iii. |
der(22)×5 |
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|
|
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|
JK-1 |
BST |
Myeloid |
i. |
3F: 9,der(22)×2[23] |
i. |
9,der(22)×2 |
i. |
22,der(9) |
|
|
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|
K562 |
PE |
Myeloid |
i. |
+5F: marker×2, der(9)t(9;17), der(9)t(9;9)[20] |
i. |
der(9)t(9;17), der(9)t(9;9) |
i. |
22×2 |
|
|
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|
KCL-22 |
PE |
Myeloid |
i. |
3F: 9,der(22)×2[20] |
i. |
9,der(22)×2 |
i. |
22,der(9) |
|
|
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|
KU812 |
PB |
Myeloid early |
i. |
2F: der(22)×2[15] |
i. |
der(22)×2 |
i. |
der(9)×2 |
|
basophilic |
ii. |
+5F: der(22), marker[9] |
ii. |
der(22), marker |
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|
|
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|
LAMA-84 |
PB |
Granulocytic, megakaryocytic and erythroid |
i. |
4F: der(22)×4[20] |
i. |
der(22)×4 |
i. |
22,der(9)×2 |
|
|
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|
MC32 |
PB |
Myeloid, lymphoid and megakaryocytoid |
i. |
2G: der(22)*,der(22)** 2F: 9,der(22)* [19] |
i. ii. |
9,der(22)* 9,der(22)**** |
i. ii. |
22,der(9) 22,der(9) |
|
ii. |
2G: der(22)*** |
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|
2F: 9,der(22)**** [3] |
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|
|
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|
MEG-01 |
BM |
Megakaryoblastic |
i. |
3F: der(22), der(15)t(15;22)[36] |
i. |
der(7)t(7;22), der(15)t(15;22) |
i. |
der(9)×2 |
|
|
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|
1 CML-T1 is a Ph negative BCR/ABL1 positive cell line. The Ph chromosomes are indeed masked Ph chromosomes with an ins(22;9)(q11.2;q34.1q34.2). CML-T1 had 3 cell clones: one diploid and two tetraploid with/without a deletion of 9q34 on the "normal" 9.2 MC3 had two Ph chromosomes with apparently classical morphology but FISH revealed the presence of two clones both with different Ph chromosome markers carrying cryptic duplications. Stars indicate the different derivative chromosomes 22. Clone i., der(22)* retained all 3' ABL1 → 9qter sequences and carried a duplication of both 9q telomere and a 438 Kb fragment from chromosome 9 (RP11-83J21 → RP11-544A12); der(22)** only retained a 682 Kb fragment from 9 (RP11-83J21 → RP11-643E14). Clone ii., der(22)*** only retained a 438 Kb region from 9 (RP11-83J21 → RP11-544A12) which was duplicated; der(22) **** retained all 3' ABL1 → 9qter sequences and carried a duplication of 9q telomere. |
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Virgili and Nacheva Molecular Cytogenetics 2010 3:15 doi:10.1186/1755-8166-3-15 |
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