Table 1

Methods and results of immunohistochemistry

Antibody

Source

Dilution/antigen retrieval

Detection method

Reactivity

CK AE1/AE3

Dako

1:200/protease digestion

SA/Bi

-

CK7

Dako

1:200/protease digestion

SA/Bi

-

CK20

Dako

1:500/protease digestion

SA/Bi

-

TTF1

Dako

1:100/CC1/95°C/30 min

SA/Bi

-

EMA

Dako

1:200/CC1/95°C/30 min

SA/Bi

-

Vimentin

Biogenex

1:10000/CC1/95°C/30 min

SA/Bi

-

CD20

Dako

1:250/CC1/95°C/30 min

SA/Bi

-

CD30

Dako

1:50/CC1/95°C/30 min

SA/Bi

-

CD34

Cell Marque

1:50/CC1/95°C/30 min

SA/Bi

-

Kappa light chain

Dako

1:5000/protease digestion

SA/Bi

-

Lambda light chain

Dako

1:10000/protease digestion

SA/Bi

-

CD68

Dako

1:800/CC1/95°C/8 min

SA/Bi

-

S-100

Univ. of Toronto

1:1000/CC1/95°C/30 min

SA/Bi

- *

HMB-45

Dako

1:100/untreated

SA/Bi

-

Melan-A

Cell Marque

1:50/CC1/95°C/30 min

SA/Bi

- *

PNL2

Private source

RTU/untreated

Polymer based

-

SMA

Dako

1:200/untreated

SA/Bi

-

Desmin

Dako

1:200/CC1/95°C/30 min

SA/Bi

+++

H-Caldesmon

Dako

1:200/CC1/95°C/30 min

SA/Bi

-

Myogenin

Dako

1:50/CC1/95°C/60 min

Polymer based

-

Myo-D1

Dako

1:60/citrate buffer (pH6.0)

Polymer based

-

CD99

Signet

1:20/CC1/95°C/30 min

SA/Bi

+++

Synaptophysin

Cell Marque

1:250/CC1/95°C/30 min

SA/Bi

+++

Fli1

BD Pharmingen

1:20/CC1/95°C/60 min

Polymer based

-

WT1

Dako

1:50/CC1/95°C/60 min

Polymer based

-

Ki 67

Lab Vision

1:200/CC1/95°C/30 min

SA/Bi

>10%

Dako, Carpinteria, CA, USA; Biogenex, San Ramon, CA, USA; Cell Marque, Rocklin, CA, USA; Signet, Dedham, MA, USA; BD Pharmingen, Franklin Lakes, NJ, USA.

SA/Bi: Streptavidin/biotin method; RTU: ready to use; CC1: Cell conditioning 1 (pH8.0) (Ventana medical systems, Tucson, AZ, USA).

*Negative on the core and open biopsies, but patchy positive staining was identified in the resection specimen.

Pacheco et al. Molecular Cytogenetics 2010 3:12   doi:10.1186/1755-8166-3-12