Figure 3.

Agarose gel of EWSR1-CREB1 RT-PCR product and sequence of the junction point. A) RT-PCR product of ~120 bp corresponding to the predicted size of the fragment spanning the break point based on the pair of primers used. M, 100 bp molecular marker; NC, negative control (molecularly confirmed clear cell sarcoma with an EWSR1-ATF1 translocation); NTC, no template control. B) Sequence electropherogram showing an EWSR1 exon7/CREB1 exon 7 in-frame fusion.