Figure 3.

Oligonucleotide microarray analysis of artificially derived mosaic trisomy 21 samples. (A) 10% trisomy 21 showing a very subtle copy-number gain for all clones on chromosome 21. The profile was generated using DNA extracted from a mixture of blood which contained 10% WBCs from a trisomy 21 subject and 90% WBCs from a normal male individual. (B) 15% trisomy 21, generated as in (A), showing a very subtle copy-number gain for all clones on chromosome 21. (C) 20% trisomy 21, generated as in (A) showing a subtle copy-number gain for all clones on chromosome 21. (D) 30% trisomy 21, generated as in (A), showing a clear copy-number gain for all clones on chromosome 21. The inset images to the right of each array plot show the average log2 ratio of all probes mapping to chromosome 21, with the horizontal dotted line representing a log2 ratio of zero and the vertical dotted line representing the centromere. A pink bar plotted above the horizontal line represents a copy-number gain of all probes on chromosome 21. To the left of each inset image is the average log2 ratio at the specified proportion of trisomic cells.

Neill et al. Molecular Cytogenetics 2010 3:11   doi:10.1186/1755-8166-3-11
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