Research

The hierarchically organized splitting of chromosomal bands for all human chromosomes

Nadezda Kosyakova , Anja Weise , Kristin Mrasek , Uwe Claussen , Thomas Liehr and Heike Nelle

Universitätsklinikum Jena, Institut für Humangenetik und Anthropologie, Jena, Germany

author email corresponding author email

Molecular Cytogenetics 2009, 2:4doi:10.1186/1755-8166-2-4

Published:	26 January 2009

Abstract

Background

Chromosome banding is widely used in cytogenetics. However, the biological nature of hierarchically organized splitting of chromosomal bands of human chromosomes is an enigma and has not been, as yet, studied.

Results

Here we present for the first time the hierarchically organized splitting of chromosomal bands in their sub-bands for all human chromosomes. To do this, array-proved multicolor banding (aMCB) probe-sets for all human chromosomes were applied to normal metaphase spreads of three different G-band levels. We confirmed for all chromosomes to be a general principle that only Giemsa-dark bands split into dark and light sub-bands, as we demonstrated previously by chromosome stretching. Thus, the biological band splitting is in > 50% of the sub-bands different than implemented by the ISCN nomenclature suggesting also a splitting of G-light bands. Locus-specific probes exemplary confirmed the results of MCB.

Conclusion

Overall, the present study enables a better understanding of chromosome architecture. The observed difference of biological and ISCN band-splitting may be an explanation why mapping data from human genome project do not always fit the cytogenetic mapping.